CD86 Mouse Anti-Rat, BUV395, Clone: 24F, BD Optibuild™

Description

• The 24F antibody reacts with CD86 (B7-2), a member of the Ig superfamily of transmembrane proteins
• CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions
• CD86 is predominantly expressed on antigen-presenting cells
• It can be upregulated on splenic B cells by in vitro polyclonal activation, such as LPS treatment
• 24F mAb is reported to block the costimulatory function of rat CD86 and to immunoprecipitate CD86 from Lewis-S1 cell lysates.HTLV-1-transformed rat T-cell line Lewis-S1
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.