CD286 (TLR6) Mouse anti-Mouse, Brilliant Violet 605, Clone: C1N2, BD Optibuild™

Description

• The C1N2 monoclonal antibody specifically binds to the Toll-like receptor 6 (TLR6), which is also known as CD286
• CD286 belongs to the mammalian toll-like family of pattern recognition receptors (TLR) that participate in inducing innate immune responses to microbial pathogens
• CD286 is a type I transmembrane glycoprotein comprised of an extracellular domain with a large number of leucine?rich repeats (LRRs) and a cytoplasmic region with a signaling Toll/Interleukin-1 Receptor (TIR) domain
• TLR6 is differentially expressed on the surface and intracellular compartments, including endolysosomes, of pre-B cells as well as immature or mature B cells
• It is likewise expressed by monocytes, macrophages, or plasmacytoid, CD4+, or CD8+ dendritic cells
• In association with TLR2 (CD282), CD286 recognizes microbial diacylated lipopeptides, lipoteichoic acid, and zymosan
• The CD282/CD286 (TLR2/TLR60 heterodimer then transduces signaling responses leading to the production of proinflammatory cytokines by responding cells
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.