CD131 Mouse anti-Human, BUV395, Clone: 3D7, BD Optibuild™

Description

• The 3D7 antibody reacts with CD131, the 120 kD commonβchain (βc) which is shared with the receptor complexes for human granulocyte-macrophage colony stimulating factor (GM-CSFR), interleukin-3 (IL-3R) and interleukin-5 (IL-5R)
• Together with theαsubunit of either the IL-3R (IL-3Rα), IL-5R (IL-5Rα), or GM- CSFR (GM-CSFRα), the commonβchain forms high-affinity, signaling receptors for human IL-3, IL-5 and GM-CSF, respectively
• Cell surfaceβc are expressed by a variety of different cell types including hematopoietic progenitor cells derived from pluripotent stem cells, monocytes, neutrophils, eosinophils, basophils, endothelial cells, fibroblasts, and Langerhans cells
• The immunogen used to generate this hybridoma was cells co-transfected with expression vectors which contained cDNA for the human IL-3αandβchains
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.