BDB743344
Marginal Zone B Cells Mouse Anti-Rat, Brilliant Violet 650, Clone: HIS57, BD Optibuild™
Manufacturer: BD Biosciences
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB743344-Each-of-1 | In Stock | ₹ 34,052.88 |
BDB743344 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 34,052.88
GST (18%): ₹ 6,129.518
Total Price: ₹ 40,182.398
Antigen
Marginal Zone B Cells
Clone
HIS57
Conjugate
Brilliant Violet 650
Host Species
Mouse
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Mouse
Isotype
IgG1 κ
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen
Rat Splenic B Cells
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Description
- The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen
- In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations
- Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM
- The HIS57 mAb does not stain granulocytes and thymocytes
- Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles
- This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat
- The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
- This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
- BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
- Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
- However, the spillover can be corrected through compensation as with any other dye combination
- BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
- Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
- BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Compare Similar Items
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Antigen: Marginal Zone B Cells
Clone: HIS57
Conjugate: Brilliant Violet 650
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Target Species: Mouse
Isotype: IgG1 κ
Classification: Monoclonal
Concentration: 0.2mg/mL
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen: Rat Splenic B Cells
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
Marginal Zone B Cells
Clone:
HIS57
Conjugate:
Brilliant Violet 650
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Mouse
Isotype:
IgG1 κ
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen:
Rat Splenic B Cells
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: Marginal Zone B Cells
Clone: HIS57
Conjugate: Brilliant Violet 711
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Target Species: Mouse
Isotype: IgG1 κ
Classification: Monoclonal
Concentration: 0.2mg/mL
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen: Rat Splenic B Cells
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
Marginal Zone B Cells
Clone:
HIS57
Conjugate:
Brilliant Violet 711
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Mouse
Isotype:
IgG1 κ
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen:
Rat Splenic B Cells
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: Marginal Zone B Cells
Clone: HIS57
Conjugate: Brilliant Ultraviolet 395
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Target Species: Mouse
Isotype: IgG1 κ
Classification: Monoclonal
Concentration: 0.2mg/mL
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen: Rat Splenic B Cells
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
Marginal Zone B Cells
Clone:
HIS57
Conjugate:
Brilliant Ultraviolet 395
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Mouse
Isotype:
IgG1 κ
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen:
Rat Splenic B Cells
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD85g (ILT7)
Clone: 17G10.2 (also known as 17.2)
Conjugate: Brilliant Violet 510
Host Species: Mouse
Purification Method: __
Regulatory Status: RUO
Target Species: Human
Isotype: IgG1 κ
Classification: Monoclonal
Concentration: __
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen: ILT7–FcεRIγ-transfected 2B4 cells
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
Antigen:
CD85g (ILT7)
Clone:
17G10.2 (also known as 17.2)
Conjugate:
Brilliant Violet 510
Host Species:
Mouse
Purification Method:
__
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG1 κ
Classification:
Monoclonal
Concentration:
__
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen:
ILT7–FcεRIγ-transfected 2B4 cells
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.