CD284 (TLR4) Mouse anti-Human, Brilliant Violet 421, Clone: HTA125, BD Optibuild™

Description

• The HTA125 monoclonal antibody specifically binds to the human Toll-like receptor 4 (TLR4), also known as CD284
• Toll-like receptors (TLR) are type I transmembrane proteins characterized by an extracellular domain containing leucine-rich repeats and a cytoplasmic domain similar to IL-1R family
• In humans, ten TLR have been identified so far
• The TLR family acts as a pattern-recognition receptor that is an essential component of pathogen recognition and innate immunity
• TLR4 has been identified as the receptor for Gram-negative bacterial lipopolysaccharide (LPS)
• TLR4 mRNA expression has been found in spleen, placenta, ovary, intestine and lung
• Using HTA125, TLR4 protein expression has been detected in monocytes, B cells and T cells in human peripheral blood
• The human TLR4 /gene has been mapped to chromosome 9q32
• The immunogen used to generate the HTA125 hybridoma was human TLR4-transfected Ba/F3 cells
• The HTA125 antibody reportedly blocks LPS-triggered, TLR4-mediated stimulation of cells
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.