TCR Vβ3 Hamster anti-Mouse, BUV395, Clone: KJ25, BD Optibuild™

Description

• The KJ25 antibody specifically reacts with the V β 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex
• V β 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 ( Mls-4[a], Mls[c] ), Mtv-3 ( Mls[c] ), Mtv-6 ( Mls-3[a], Mls[c] ), Mtv-13 ( Mls-2[a], Mls[c] ), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW)
• V β 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B
• Activation or elimination of V β 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E
• This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.