CD201 Rat anti-Human, Brilliant Violet 605, Clone: RCR-252, BD Optibuild™

Description

• The RCR-252 monoclonal antibody specifically binds to the endothelial cell protein C receptor (EPCR), which is also known as CD201
• CD201 is a 25 kDa, type I transmembrane glycoprotein, expressed on endothelial cells in arteries, veins and capillaries
• CD201 binds activated protein C, a serine protease that is involved in regulating blood coagulation
• EPCR is reportedly expressed on several malignant cell lines
• Malignant cell lines that express EPCR can show significant levels of protein C activation and this activation can be inhibited by anti-EPCR antiboy
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.