Vβ 11 T-Cell Receptor Rat anti-Mouse, Brilliant Violet 421, Clone: RR3-15, BD Optibuild™

Description

• The RR3-15 antibody reacts with the Vβ11 T-Cell Receptor (TCR) of mice having the b haplotype (e.g., A, C57BL, C58, DBA/1) of the Tcrb gene complex
• The Tcrb-V11 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) and c (e.g., RIII) haplotypes
• VβTCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-9 (Etc-1, Mls[f], Dvb11.2) and/or Mtv-11 (Mls[f], Dvb 11.2) proviruses (e.g., AKR, BALB/c, CBA/J, C3H, DBA/2), and they are incompletely eliminated in mice expressing I-E and Mtv-8 (Mls[f], Dvb 11.1) superantigen (e.g., A)
• Activation of Vβ11 TCR-expressing T cells by these determinants is dependent upon presentation by I-E
• The bacterial superantigen Staphylococcal enterotoxin A (SEA) also interacts with Vβ11 TCR, and in vivo exposure to SEA causes activation and subsequent deletion of VβTCR-expressing lymphocytes
• Plate-bound RR3-15 antibody activates Vβ11 TCR-bearing T cells
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.