CD74 Mouse anti-Human, Brilliant Violet 421, Clone: LN2 (also known as LN-2), BD Optibuild™

Description

• The LN2 monoclonal antibody specifically recognizes CD74, the cell surface MHC class II-associated invariant chain molecule (41, 35, 33 kDa)
• CD74 is moderately expressed on B cells, and weakly on monocytes and macrophages
• Other reactive cells include Langerhans cells, dendritic cells, a subset of activated T cells and thymic epithelium
• The LN2 monoclonal antibody labels a nuclear membrane protein of follicular center and mantle zone B cells and a cytoplasmic protein of cells which bind HLA class II antibodies
• CD74 is postulated to play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface
• This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections without citrate pretreatment
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.