Vδ2 TCR Mouse anti-Human, Brilliant Violet 605, Clone: B6, BD Optibuild™

Description

• The B6 monoclonal antibody specifically recognizes the human variableδ2 subunit (Vδ2) of theγ/δT cell receptor (TCR)
• Theγ/δTCR is composed of two disulfide linked glycoproteins
• The delta chain is approximately 40-60 kDa and is restricted to peripheral blood T cells and thymocytes
• The majority of normal peripheral bloodγ/δT cells express a Vγ9[+]/ Vδ2[+] phenotype
• The reason for this selection in the T-cell repertoire is not well understood
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.