CD44 Mouse Anti-Rat, Brilliant Violet 421, Clone: OX-49, BD Optibuild™

Description

• The OX-49 antibody reacts with the glycoprotein CD44H (also known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes, and at greatly increased levels on T- and B-cell blasts
• The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44
• However, recent reports indicate that OX-49 antibody cannot detect the CD44v isoform, possibly due to conformational changes in the epitope
• CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.