RT1B Mouse anti-Rat, Brilliant Violet 421, Clone: OX-6, BD Optibuild™

Description

• The OX-6 antibody specifically recognizes non-polymorphic determinants of the Rat MHC class II antigen, I-A equivalent
• RT1B is found on peripheral B lymphocytes, thymic cortical epithelial and medullary reticular cells, small intestinal villus epithelium, epidermal Langerhans cells, dendritic cells, some tissue macrophage populations, peritoneal mast cells, and a subset of thymocytes, but not on peripheral T cells, erythrocytes, or microglia
• The OX-6 mAb cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A[g7]) mice
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.