NCAM-1 (CD56) Mouse anti-Human, BUV395, Clone: R19-760, BD Optibuild™

Description

• The R19-760 monoclonal antibody specifically binds to NCAM-1 (Neural cell adhesion molecule 1) that is also known as CD56 and NKH1
• NCAM-1 is a heavily glycosylated transmembrane protein and a member of the Ig supergene family
• NCAM-1 represents an isoform of the neural cell adhesion molecule (NCAM)
• In the hematopoietic system, it is present on approximately 10% to 25% of peripheral blood lymphocytes
• It is expressed on natural killer (NK) cells and a subset of T cells, NKT cells
• It is not expressed on myeloid cells, erythrocytes or B cells
• This molecule appears to function as an adhesion molecule and can serve as a panspecific NK-cell marker
• The R19-760 antibody recognizes an epitope in the NCAM-1 (CD56) extracellular domain that resists destruction due to cellular fixation with BD Phosflow™ Lyse/Fix Buffer and permeabilization with BD Phosflow™ Perm Buffer III
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.