Vβ 6 T-Cell Receptor Rat anti-Mouse, Brilliant Violet 605, Clone: RR4-7, BD Optibuild™

Description

• The RR4-7 antibody specifically reacts with the V β 6 T-Cell Receptor (TCR) of mice having the a ( e.g., C57BR, C57L, SJL) and b ( e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the Tcrb gene complex
• The Tcrb-V6 gene locus is deleted in mice having the c ( e.g., RIII) haplotype
• V β 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by Mtv-7 ( Mls-1[a], Mls[a] ) endogenous provirus ( e.g., AKR, CBA/J, C58, DBA/2, NZB), or Mtv -43 endogenous provirus ( e.g., MA/MyJ)
• Exogenous MMTV-SW, as well as endogenous Mtv -44-encoded superantigen ( e.g., NZW), also causes incomplete elimination of V β 6 TCR-expressing T cells
• Plate-bound RR4-7 antibody activates V β 6 TCR-bearing T cells, soluble RR4-7 mAb blocks in vitro proliferation and cytolytic activities of V β 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of V β 6 TCR-bearing T cells
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.