EPCAM (CD326) Mouse, anti-Human, R718, Clone: KS1/4, BD OptiBuild™

Description

• Description KS1/4 antigen (Ag), defined by the KS1/4 antibody (KS1/4), is expressed in many epithelial-derived carcinomas and normal epithelial cell surfaces
• The cDNA encoding KS1/4 has been isolated and contains an open reading frame of 314 amino acids including a putative signal sequence
• KS1/4 Ag migrates as three glycosylated polypeptides with molecular masses of 35, 40 and 42 kDa
• The 40 and 42 kDa species are similar proteins and appear to differ only by their degree of N-linked glycosylation
• The 35 kDa species results from proteolytic cleavages of the larger molecular weight proteins
• KS1/4 was first described as a monoclonal antibody which recognized a lung adenocarcinoma associated antigen
• In oncolytic drug targeting studies, KS1/4 suppressed the growth of human lung tumor xenografts in athymic nude mice
• Subsequent studies showed that KS1/4 reacts with a variety of tumor tissues including colon, breast, ovarian, and pancreas
• It also recognizes normal epithelium including colon, stomach, small intestine, liver, kidney, lung, pancreas, skin and ovary
• These results suggest that KS1/4 Ag represents an epithelial cell/epithelial-derived carcinoma marker
• KS1/4 recognizes a 40 - 42 kDa antigen expressed on the cell surfaces of a variety of epithelial tumors and normal epithelial cell types
• KS1/4 also recognizes a 35 kDa proteolytic fragment
• UCLA-P3 cells derived from a human adenocarcinoma of the lung were used as immunogen.