CD95 (APO-1/Fas) Monoclonal Antibody (APO-1-1), eBioscience™, Invitrogen™

Description

• Description: Immunohistochemistry: APO-1-1 (BMS151, IgG1) is an isotype-switch variant of the original clone APO-1-3 (IgG3)
• Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, BMS151 is especially useful for immunohistology on cryostat sections and on cytospin preparations
• Flow cytometry: BMS151 is also suitable as primary antibody in staining for FACS analysis, BMS151FI can be used for direct staining of cells, BMS151BT can be used in a two-step procedure using the biotin-(strept-)avidin system
• Recommended concentration for the unlabelled antibody is 1 - 2 μg/mL and a 1:20 dilution of the labelled antibodies, as determined on SKW6.4 cells
• Applications Tested: Flow Cytometry, Immunohistochemistry
• Purity: >95%
• FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily
• FAS also exists in a soluble form that weight approximately 26kDa
• Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases
• The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis
• FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells
• At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described
• The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform
• The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer
• It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.