IL-6 Monoclonal Antibody (32C11), Invitrogen™

Description

• MM600D targets IL-6 in ELISA, and WB applications and shows reactivity with mouse samples
• The MM600D immunogen is recombinant mouse IL-6
• MM600D detects IL-6 which has a predicted molecular weight of approximately 22 kDa
• The MM600D mouse IL6 antibody (clone 32C11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM600CB (biotinylated conjugate of clone 20F3)
• Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection
• Antibody MM600D (clone 32C11) and biotinylated antibody MM600CB (clone 20F3) have successfully been used in combination with recombinant mouse IL6 protein SMIL6 in ELISA applications
• This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice
• The resulting ascites is collected from the mice and the antibody is purified
• This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg)
• IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells
• The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections
• The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha
• The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis
• Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2)
• Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.