CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), NovaFluor™ Blue 760, eBioscience™, Invitrogen™

Description

• CD62L (L-Selectin) Monoclonal antibody specifically detects CD62L (L-Selectin) in Human samples
• It is validated for Flow Cytometry Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family
• CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1
• These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues
• Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis
• Applications Tested: The DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.4 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer
• NovaFluor dyes are not compatible with DNA intercalating viability dyes
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI
• Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
• This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat
• No
• (<a href='https://www.thermofisher.com/order/catalog/product/C001T06F01'>C001T06F01</a>))
• This buffer contains formulation improvements over CellBlox
• CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used
• Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells
• Add 5 μL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
• For single-color controls, use 5 μL of CellBlox Blocking Buffer per 100 μL of cell sample containing 10^3 to 10^8 cells
• NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts
• <a href = https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/novafluor-dyes.html>Learn more </a> Excitation: 492 nm; Emission: 766 nm; Laser: 488 nm (Blue) Laser