CD54 (ICAM-1) Monoclonal Antibody (HA58), NovaFluor™ Red 685, eBioscience™, Invitrogen™

Description

• The HA58 mAb reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells
• Expression of CD54 is upregulated on activated lymphocytes
• Interaction of CD54 with its ligand CD11a is important in the inflammatory response
• The HA58 antibody has been reported for use in flow cytometric analysis
• The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 5 uL (0.4 ug) per test
• A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• NovaFluor dyes are not compatible with DNA intercalating viability dyes
• Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
• Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer
• Add 5 uL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
• For single-color controls, use 5 uL of CellBlox Blocking Buffer per 100 uL of cell sample containing 10^3 to 10^8 cells
• Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser CD54 (ICAM-1) Monoclonal antibody specifically detects CD54 (ICAM-1) in Human samples
• It is validated for Flow Cytometry