CD8a Monoclonal Antibody (53-6.7), NovaFluor™ Blue 660-120S, eBioscience™, Invitrogen™

Description

• The 53-6.7 mAb reacts with the mouse CD8a molecule
• CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha)
• A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha
• CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells
• This 53-6.7 antibody has been reported for use in flow cytometric analysis
• This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5 ug per test
• A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• NovaFluor dyes are not compatible with DNA intercalating viability dyes
• Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
• Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer CD8a Monoclonal antibody specifically detects CD8a in Mouse samples
• It is validated for Flow Cytometry