CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), NovaFluor™ Blue 760, eBioscience™, Invitrogen™

Description

• CD90.2 (Thy-1.2) Monoclonal antibody specifically detects CD90.2 (Thy-1.2) in Mouse samples
• It is validated for Flow Cytometry Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule
• CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains
• These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others
• Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1
• CD90 is involved in regulation of adhesion and signal transduction by T cells
• Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.4 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer
• NovaFluor dyes are not compatible with DNA intercalating viability dyes
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI
• Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
• This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat
• No
• (<a href='https://www.thermofisher.com/order/catalog/product/C001T06F01'>C001T06F01</a>))
• This buffer contains formulation improvements over CellBlox
• CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used
• Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells
• Add 5 μL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
• For single-color controls, use 5 μL of CellBlox Blocking Buffer per 100 μL of cell sample containing 10^3 to 10^8 cells
• NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts
• <a href = https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/novafluor-dyes.html>Learn more </a> Excitation: 492 nm; Emission: 766 nm; Laser: 488 nm (Blue) Laser