RTN1 Monoclonal Antibody (RNL-3), Invitrogen™

Description

• MA1-06814 detects reticulon-1A(NSP-A) and reticulon-1B(NSP-B) in human, rabbit, and non-human primate cells
• MA1-06814 has sucessfully been used in Western blotting, immunocytochemistry, FACS and immunohistochemistry (paraffin) procedures
• By Western blot it detects a ∽135 kDa band representing reticulon-1A, and a ∽45 kDa band representing reticulon-1B
• The MA1-06814 immunogen is a small cell lung cancer cell line NCI-H82
• Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues
• The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22
• The NSP-gene encodes three overlapping proteins, i.e
• reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C (NSP-C)
• These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions
• Reticulon-1A is a protein with a molecular weight of approximately 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues
• In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A
• Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82
• Reticulon-1B is so far not found in human tissues
• Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).