HLA-ABC Monoclonal Antibody (W6/32), Invitrogen™

Description

• MA1-70111 detects HLA-ABC in human samples
• MA1-70111 has been successfully used in flow cytometry, functional assay, and immunoprecipitation procedures
• The MA1-70111 immunogen is purified membrane of human tonsil cells
• Store at 4°C short term
• For extended storage aliquot and store at -20°C
• Avoid Freeze/Thaw Cycles
• HLA-A belongs to the HLA class I heavy chain paralogues
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin)
• The heavy chain is anchored in the membrane
• Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen
• They are expressed in nearly all cells
• The heavy chain is approximately 45 kDa and its gene contains 8 exons
• Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail
• Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule
• Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation
• Hundreds of HLA-A alleles have been described.