MABT1353100
C-Nap1 (Cep250), Mouse anti-Human, Clone: 150-230-1, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT1353100-Each-of-1 | In Stock | ₹ 41,308.37 |
MABT1353100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,308.37
GST (18%): ₹ 7,435.507
Total Price: ₹ 48,743.877
Antigen
C-Nap1 (Cep250)
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
CEP250;CEP2;CNAP1
Immunogen
Recombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_001305148
Target Species
Human
Form
Purified
Applications
Immunofluorescence, Western Blot
Clone
150-230-1
Dilution
Immunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Gene Alias
Centrosome-associated protein CEP250;250 kDa centrosomal protein;Cep250;Centrosomal Nek2-associated protein 1;C-Nap1;Centrosomal protein 2
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Description
- Anti-C-Nap1 (Cep250), clone 150-230-1, Cat
- No
- MABT1353, is a highly specific mouse monoclonal antibody that targets Centrosome-associated protein CEP250 and has been tested for use in Immunofluorescence and Western Blotting
- Centrosome-associated protein CEP250 (UniProt: Q9BV73; also known as 250 kDa centrosomal protein, Cep250, Centrosomal NEK2-associated protein 1, C-Nap1, Centrosomal protein 2) is encoded by the CEP250 (also known as CEP2, CNAP1) gene (Gene ID: 11190) inn human
- Cep250 is a component of the core centrosome that is ubiquitously expressed
- It can exist as a monomer and homodimer
- It specifically associates with the proximal ends of both mother and daughter centrioles during interphase and in mitotic cells it dissociates from the mitotic spindle poles
- At the end of cell division, it is shown to re-accumulate at centrosomes
- Cep250 is differentially phosphorylated during cell cycle and phosphorylation status regulates its association/dissociation from centrosome
- During M phase its C-terminal part is shown to be phosphorylated by NEK2
- Two isoforms of Cep250 have been described that are produced by alternative splicing
- Loss of Cep250-mediated centriole cohesion leads to an altered cell migration phenotype.
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Antigen: C-Nap1 (Cep250)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: CEP250;CEP2;CNAP1
Immunogen: Recombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_001305148
Target Species: Human
Form: Purified
Applications: Immunofluorescence, Western Blot
Clone: 150-230-1
Dilution: Immunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Gene Alias: Centrosome-associated protein CEP250;250 kDa centrosomal protein;Cep250;Centrosomal Nek2-associated protein 1;C-Nap1;Centrosomal protein 2
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
C-Nap1 (Cep250)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
CEP250;CEP2;CNAP1
Immunogen:
Recombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_001305148
Target Species:
Human
Form:
Purified
Applications:
Immunofluorescence, Western Blot
Clone:
150-230-1
Dilution:
Immunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Gene Alias:
Centrosome-associated protein CEP250;250 kDa centrosomal protein;Cep250;Centrosomal Nek2-associated protein 1;C-Nap1;Centrosomal protein 2
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: GM130
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Golga2
Immunogen: A full length recombinant human Golgin subfamily A member 2 (GM130).
Quantity: 25 μL
Research Discipline: Cell Structure
Gene ID (Entrez): __
Target Species: Rat, Human
Form: Purified
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone: 4A3
Dilution: Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GM130 in human liver cancer tissue.Western Blotting Analysis: A representative lot detected GM130 in Western Blotting applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12).Immunocytochemistry Analysis: A representative lot detected GM130 in Immunocytochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51; Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12).Immunohistochemistry Analysis: A representative lot detected GM130 in Immunohistochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51).Immunoprecipitation Analysis: A representative lot detected GM130 in Immunoprecipitation applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12; Diao, A., et. al. (2008) J Cell Biol. 283(11):6957-67).
Gene Alias: Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 4A3 detected Golgin subfamily A member 2 (GM130) in human liver cancer and human intestine sections.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Antigen:
GM130
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Golga2
Immunogen:
A full length recombinant human Golgin subfamily A member 2 (GM130).
Quantity:
25 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
__
Target Species:
Rat, Human
Form:
Purified
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone:
4A3
Dilution:
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GM130 in human liver cancer tissue.Western Blotting Analysis: A representative lot detected GM130 in Western Blotting applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12).Immunocytochemistry Analysis: A representative lot detected GM130 in Immunocytochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51; Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12).Immunohistochemistry Analysis: A representative lot detected GM130 in Immunohistochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51).Immunoprecipitation Analysis: A representative lot detected GM130 in Immunoprecipitation applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12; Diao, A., et. al. (2008) J Cell Biol. 283(11):6957-67).
Gene Alias:
Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 4A3 detected Golgin subfamily A member 2 (GM130) in human liver cancer and human intestine sections.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2b κ
Antigen: Tropomyosin-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: TPM1;C15orf13;TMSA
Immunogen: KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_000357.3
Target Species: Mouse, Human
Form: Purified
Applications: Immunocytochemistry, Western Blot
Clone: 7F8B8
Dilution: Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Gene Alias: Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Antigen:
Tropomyosin-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
TPM1;C15orf13;TMSA
Immunogen:
KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_000357.3
Target Species:
Mouse, Human
Form:
Purified
Applications:
Immunocytochemistry, Western Blot
Clone:
7F8B8
Dilution:
Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Gene Alias:
Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Antigen: Tropomyosin-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: TPM1;C15orf13;TMSA
Immunogen: KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Quantity: 25 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_000357.3
Target Species: Mouse, Human
Form: Purified
Applications: Immunocytochemistry, Western Blot
Clone: 7F8B8
Dilution: Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Gene Alias: Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Antigen:
Tropomyosin-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
TPM1;C15orf13;TMSA
Immunogen:
KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Quantity:
25 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_000357.3
Target Species:
Mouse, Human
Form:
Purified
Applications:
Immunocytochemistry, Western Blot
Clone:
7F8B8
Dilution:
Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Gene Alias:
Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ