Podoplanin, Mouse anti-Human, Clone: LpMab-19, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT85325UG-Each-of-1 | In Stock | ₹ 16,456.61 |
MABT85325UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
Podoplanin
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
PDPN;GP36
Immunogen
LN229 glioma cells expressiing human Podoplanin.
Quantity
25 μg
Research Discipline
Cell Structure
Test Specificity
Clone LpMab-19 detects Podoplanin in human cells. It recognizes sialylated O-Glycan on Thr76. The minimal epitope of LpMab-19 is identified as Thr76-Arg79.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2b κ
Applications
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone
LpMab-19
Dilution
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in human colon cancer tissue. ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
Gene Alias
Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Description
- Anti-Podoplanin, clone LpMab-19, Cat
- No
- MABT853, is a mouse monoclonal antibody that detects human Podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin)
- Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human
- Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes
- A high degree of expression is observed in placenta, lung, skeletal muscle, and brain
- It may be involved in cell migration and/or actin cytoskeleton organization
- Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles
- Six isoforms of podoplanin have been described that are produced by alternative splicing
- It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form
- Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162)
- Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus
- Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2)
- Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3
- Clone LpMab-19 recognizes Sialylated O-Glycan on Thr76 of human Podoplanin
- Its reactivity with Podoplanin is lost following T76A mutation
- LpMab-19 recognizes endogenous hPDPN that is expressed in LN319 glioblastoma cell line, but fails to react with LN319 cells with hPDPN knock out
- The minimum epitope of LpMab-19 is identified to be Thr76-Arg79 hPDPN
- (Ref.: Ogasawara, S., et al
- (2016)
- Monoclon
- Antib
- immunodiag
- Immunother
- 35(5); 245-253).
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Antigen: Podoplanin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: PDPN;GP36
Immunogen: LN229 glioma cells expressiing human Podoplanin.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone LpMab-19 detects Podoplanin in human cells. It recognizes sialylated O-Glycan on Thr76. The minimal epitope of LpMab-19 is identified as Thr76-Arg79.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone: LpMab-19
Dilution: Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in human colon cancer tissue. ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
Gene Alias: Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
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