NB007378

EMI1 Antibody (EMI1/1176), Alexa Fluor™ 594, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NB007378-Each-of-1 In Stock ₹ 53,688.90

NB007378 - Each of 1

₹ 53,688.90

In Stock

Quantity

1

Base Price: ₹ 53,688.90

GST (18%): ₹ 9,664.002

Total Price: ₹ 63,352.902

Antigen

EMI1

Classification

Monoclonal

Conjugate

Alexa Fluor 594

Formulation

50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols

FBXO5

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)

Quantity

0.1 mL

Primary or Secondary

Primary

Target Species

Human

Isotype

IgG2a κ

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone

EMI1/1176

Dilution

Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias

EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Host Species

Mouse

Purification Method

Protein A or G purified

Regulatory Status

RUO

Test Specificity

It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Content And Storage

Store at 4°C in the dark.

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Description

  • EMI1 Monoclonal specifically detects EMI1 in Human samples
  • It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

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Img

Novus Biologicals

NB007378

--


Antigen:
EMI1

Classification:
Monoclonal

Conjugate:
Alexa Fluor 594

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
FBXO5

Immunogen:
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
EMI1/1176

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NB007380

--


Antigen:
EMI1

Classification:
Monoclonal

Conjugate:
DyLight 594

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
FBXO5

Immunogen:
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
EMI1/1176

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NB007381

--


Antigen:
Golgi Glycoprotein 1/GLG1

Classification:
Monoclonal

Conjugate:
Alexa Fluor 350

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
GLG1

Immunogen:
Golgi fraction from human liver cells

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human, Mouse (Negative), Rat (Negative)

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
GLG1/970

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry

Gene Alias:
CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This monoclonal antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NB007382

--


Antigen:
Golgi Glycoprotein 1/GLG1

Classification:
Monoclonal

Conjugate:
Alexa Fluor 532

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
GLG1

Immunogen:
Golgi fraction from human liver cells

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human, Mouse (Negative), Rat (Negative)

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
GLG1/970

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry

Gene Alias:
CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This monoclonal antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membra

Content And Storage:
Store at 4°C in the dark.