Classification:
Monoclonal
Immunogen:
This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
Research Discipline:
Angiogenesis, Cancer, Chromatin Research, Core ESC Like Genes, Epigenetics, Phospho Specific, Stem Cell Markers, Transcription Factors and Regulators
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Sheep
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown
Dilution:
Western Blot : 0.1 - 0.5 ug/ml, Simple Western 1:100, Flow Cytometry : 1 - 5 ug/ml, Immunohistochemistry : 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:10. Use reported in scientific literature (PMID 24386225), Immunoprecipitation : 1:10 - 1:500, Immunohistochemistry-Paraffin : 1:50 - 1:500, Immunohistochemistry-Frozen : 1:500, Flow (Intracellular) reported in scientific literature (PMID 24578214), Immunohistochemistry Free-Floating : 1:500, Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready, Knockdown Validated
Gene Alias:
AIM, CXXC9CXXC finger protein 9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, Dnmt1, EC 2.1.1.37, FLJ16293, m.HsaI, MCMTMGC104992
Purification Method:
Protein G purified
Primary or Secondary:
Primary
Test Specificity:
Rat reactivity reported in scientific literature (PMID: 19924110). Porcine reactivity reported in scientific literature (PMID: 23808878). Sheep reactivity reported in scientific literature (PMID: 20413592). Bovine reactivity reported in scientific literature (PMID:27384909).
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.