CD40/TNFRSF5 Antibody (5D12), PerCP, Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB016178-Each-of-1 | In Stock | ₹ 55,271.76 |
NB016178 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,271.76
GST (18%): ₹ 9,948.917
Total Price: ₹ 65,220.677
Antigen
CD40/TNFRSF5
Classification
Monoclonal
Conjugate
PerCP
Formulation
PBS
Gene Symbols
CD40
Immunogen
Anti-CD40/TNFRSF5 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40/TNFRSF5 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
Quantity
0.1 mL
Research Discipline
Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
Gene ID (Entrez)
958
Target Species
Human, Cynomolgus Monkey
Form
Purified
Applications
Flow Cytometry
Clone
5D12
Dilution
Flow Cytometry
Gene Alias
B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
Host Species
Human
Purification Method
Protein A purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
ch5D12 binds specifically to recombinant and native human CD40/TNFRSF5 ectodomain and has been shown to also bind CD40/TNFRSF5 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis.
Content And Storage
Store at 4°C in the dark.
Isotype
IgG4 κ
Related Products
Description
- CD40/TNFRSF5 Monoclonal specifically detects CD40/TNFRSF5 in Human, Cynomolgus Monkey samples
- It is validated for Flow Cytometry.
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Antigen: CD40/TNFRSF5
Classification: Monoclonal
Conjugate: PerCP
Formulation: PBS
Gene Symbols: CD40
Immunogen: Anti-CD40/TNFRSF5 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40/TNFRSF5 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
Quantity: 0.1 mL
Research Discipline: Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
Gene ID (Entrez): 958
Target Species: Human, Cynomolgus Monkey
Form: Purified
Applications: Flow Cytometry
Clone: 5D12
Dilution: Flow Cytometry
Gene Alias: B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
Host Species: Human
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: ch5D12 binds specifically to recombinant and native human CD40/TNFRSF5 ectodomain and has been shown to also bind CD40/TNFRSF5 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG4 κ
Antigen: Lymphotoxin-alpha/TNF-beta
Classification: Monoclonal
Conjugate: Alexa Fluor 750
Formulation: 50mM Sodium Borate
Gene Symbols: LTA
Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Quantity: 0.1 mL
Research Discipline: Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Gene ID (Entrez): 4049
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA
Clone: 3F12.2D3
Dilution: Western Blot, Flow Cytometry, ELISA
Gene Alias: LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Host Species: Rabbit
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: 3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG κ
Antigen: Lymphotoxin-alpha/TNF-beta
Classification: Monoclonal
Conjugate: Biotin
Formulation: PBS
Gene Symbols: LTA
Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Quantity: 0.1 mL
Research Discipline: Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
Gene ID (Entrez): 4049
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA
Clone: 3F12.2D3
Dilution: Western Blot, Flow Cytometry, ELISA
Gene Alias: LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
Host Species: Rabbit
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: 3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG κ