NB016370
Fas/TNFRSF6/CD95 Antibody (R-125224), DyLight 350, Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB016370-Each-of-1 | In Stock | ₹ 55,271.76 |
NB016370 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,271.76
GST (18%): ₹ 9,948.917
Total Price: ₹ 65,220.677
Antigen
Fas/TNFRSF6/CD95
Classification
Monoclonal
Conjugate
DyLight 350
Formulation
50mM Sodium Borate
Gene Symbols
FAS
Immunogen
R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity
0.1 mL
Research Discipline
Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez)
355
Target Species
Human
Form
Purified
Applications
Flow Cytometry, ELISA
Clone
R-125224
Dilution
Flow Cytometry, ELISA
Gene Alias
Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species
Human
Purification Method
Protein A purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage
Store at 4°C in the dark.
Isotype
IgG1 κ
Related Products
Description
- Fas/TNFRSF6/CD95 Monoclonal specifically detects Fas/TNFRSF6/CD95 in Human samples
- It is validated for Flow Cytometry, ELISA.
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Antigen: Fas/TNFRSF6/CD95
Classification: Monoclonal
Conjugate: DyLight 350
Formulation: 50mM Sodium Borate
Gene Symbols: FAS
Immunogen: R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity: 0.1 mL
Research Discipline: Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez): 355
Target Species: Human
Form: Purified
Applications: Flow Cytometry, ELISA
Clone: R-125224
Dilution: Flow Cytometry, ELISA
Gene Alias: Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species: Human
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG1 κ
Antigen:
Fas/TNFRSF6/CD95
Classification:
Monoclonal
Conjugate:
DyLight 350
Formulation:
50mM Sodium Borate
Gene Symbols:
FAS
Immunogen:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity:
0.1 mL
Research Discipline:
Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez):
355
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, ELISA
Clone:
R-125224
Dilution:
Flow Cytometry, ELISA
Gene Alias:
Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species:
Human
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG1 κ
Antigen: Fas/TNFRSF6/CD95
Classification: Monoclonal
Conjugate: Alexa Fluor 350
Formulation: 50mM Sodium Borate
Gene Symbols: FAS
Immunogen: R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity: 0.1 mL
Research Discipline: Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez): 355
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone: R-125224
Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence
Gene Alias: Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species: Rabbit
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG κ
Antigen:
Fas/TNFRSF6/CD95
Classification:
Monoclonal
Conjugate:
Alexa Fluor 350
Formulation:
50mM Sodium Borate
Gene Symbols:
FAS
Immunogen:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity:
0.1 mL
Research Discipline:
Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez):
355
Target Species:
Human
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone:
R-125224
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence
Gene Alias:
Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species:
Rabbit
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG κ
Antigen: Fas/TNFRSF6/CD95
Classification: Monoclonal
Conjugate: Alexa Fluor 405
Formulation: 50mM Sodium Borate
Gene Symbols: FAS
Immunogen: R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity: 0.1 mL
Research Discipline: Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez): 355
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone: R-125224
Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence
Gene Alias: Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species: Rabbit
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG κ
Antigen:
Fas/TNFRSF6/CD95
Classification:
Monoclonal
Conjugate:
Alexa Fluor 405
Formulation:
50mM Sodium Borate
Gene Symbols:
FAS
Immunogen:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity:
0.1 mL
Research Discipline:
Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez):
355
Target Species:
Human
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone:
R-125224
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence
Gene Alias:
Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species:
Rabbit
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG κ
Antigen: Fas/TNFRSF6/CD95
Classification: Monoclonal
Conjugate: Alexa Fluor 488
Formulation: 50mM Sodium Borate
Gene Symbols: FAS
Immunogen: R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity: 0.1 mL
Research Discipline: Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez): 355
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone: R-125224
Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence
Gene Alias: Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species: Rabbit
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage: Store at 4°C in the dark.
Isotype: IgG κ
Antigen:
Fas/TNFRSF6/CD95
Classification:
Monoclonal
Conjugate:
Alexa Fluor 488
Formulation:
50mM Sodium Borate
Gene Symbols:
FAS
Immunogen:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells.
Quantity:
0.1 mL
Research Discipline:
Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
Gene ID (Entrez):
355
Target Species:
Human
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone:
R-125224
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence
Gene Alias:
Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6
Host Species:
Rabbit
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG κ