Antigen:
Paramyxovirus SV5 Pk
Classification:
Monoclonal
Formulation:
PBS with 0.09% Sodium Azide
Gene Alias:
Simian virus 5; SPV5gp2; V protein
Purification Method:
Protein G purified
Primary or Secondary:
Primary
Test Specificity:
This product recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NB100-62264 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro (ile pro asn pro leu leu gly leu asp) ser thr. (9 amino acid epitope is in parenthesis)
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Applications:
Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, ELISA, Western Blot
Dilution:
Western Blot 1:1000-1:5000, Flow Cytometry 1:10-1:1000, ELISA 1:1000-1:5000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000, Immunohistochemistry-Frozen 1:10-1:500, Radioimmunoassay 1:100-1:2000, Sandwich ELISA
Immunogen:
Mice were infected with the paramyxovirus SV5, Simian-Virus 5
Research Discipline:
Epitope Tags, V5 Epitope Tags
Target Species:
Virus, Mouse