Home Products Antibodies Primary Antibodies NB019477
NB019477

Astrocytomas Antibody (J1-31), Novus Biologicals™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 NB019477-Each-of-1 In Stock ₹ 17,454.24

NB019477 - Each of 1

₹ 17,454.24

In Stock

Quantity

1

Base Price: ₹ 17,454.24

GST (18%): ₹ 3,141.763

Total Price: ₹ 20,596.003

Antigen

Astrocytomas

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

Ascites with No Preservative

Host Species

Mouse

Purification Method

Unpurified

Regulatory Status

RUO

Test Specificity

The antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed). Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP (Malhotra, SK (1989) J Neurosci Res. 22(1):36-49).

Content And Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Isotype

IgM

Applications

Immunocytochemistry, Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence

Clone

J1-31

Dilution

Western Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500

Gene Alias

Astrocytoma Marker, Astrocytomas Marker, Low Grade Astrocytoma Marker

Immunogen

Human cerebral white matter plaque materials from a multiple sclerosis patient.

Quantity

0.025 mL

Primary or Secondary

Primary

Target Species

Human, Rat

Form

Ascites

Description

  • Astrocytomas Monoclonal specifically detects Astrocytomas in Human, Rat samples
  • It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Compare Similar Items

Show Difference

Img

Fischer Scientific

NB019477

--

Antigen:
Astrocytomas
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Ascites with No Preservative
Host Species:
Mouse
Purification Method:
Unpurified
Regulatory Status:
RUO
Test Specificity:
The antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed). Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP (Malhotra, SK (1989) J Neurosci Res. 22(1):36-49).
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype:
IgM
Applications:
Immunocytochemistry, Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
J1-31
Dilution:
Western Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Gene Alias:
Astrocytoma Marker, Astrocytomas Marker, Low Grade Astrocytoma Marker
Immunogen:
Human cerebral white matter plaque materials from a multiple sclerosis patient.
Quantity:
0.025 mL
Primary or Secondary:
Primary
Target Species:
Human, Rat
Form:
Ascites

Img

Fischer Scientific

NB019488

--

Antigen:
Opsin 1 (Medium Wave)
Classification:
Polyclonal
Conjugate:
Unconjugated
Formulation:
PBS with 0.02% Sodium Azide
Host Species:
Chicken
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Test Specificity:
Opsin, red and green. The antibody reacts with a protein of ∼40 kDa.
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype:
__
Applications:
ELISA, Western Blot
Clone:
__
Dilution:
Western Blot 1:500:1:2000, ELISA 1:100-1:2000
Gene Alias:
GCP, GOP, Green cone photoreceptor pigment, Green-sensitive opsin, medium-wave-sensitive opsin 1, OPN1MW, opsin 1 (cone pigments), medium-wave-sensitive 2
Immunogen:
Synthetic peptides from mouse green opsin and zebra finch red opsin.
Quantity:
0.025 mg
Primary or Secondary:
Primary
Target Species:
Rat, Mouse
Form:
__

Img

Fischer Scientific

NB019489

--

Antigen:
LBX1
Classification:
Polyclonal
Conjugate:
Unconjugated
Formulation:
PBS with 0.1% Sodium Azide
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Test Specificity:
Lbx1 (Ladybird). By Western Blot the antibody recognizes a band at ∼30 kDa on mouse spinal cord lysate.
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype:
IgG
Applications:
Western Blot
Clone:
__
Dilution:
Western Blot 1:500-1:1000
Gene Alias:
homeobox, HPX6, HPX-6, ladybird homeobox 1, ladybird homeobox homolog 1, ladybird homeobox homolog 1 (Drosophila), Ladybird homeobox protein homolog 1, LBX1Hlady bird-like homeobox, transcription factor LBX1, transcription factor similar to D. melanogaster homeodomain protein lady birdlate
Immunogen:
Synthetic peptide.
Quantity:
0.025 mg
Primary or Secondary:
Primary
Target Species:
Mouse
Form:
__

Img

Fischer Scientific

NB019490

--

Antigen:
NMDAR2A
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Test Specificity:
Amino acid region 1099-1213 from human NMDAR2A produced as a GST fusion protein.
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype:
IgG1
Applications:
Immunofluorescence, Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blot, Immunohistochemistry
Clone:
T7
Dilution:
Western Blot 1-5 ug/ml, Immunohistochemistry 25-5 ug/ml, Immunocytochemistry/Immunofluorescence 25-50ug/ml, Immunohistochemistry-Paraffin 1:10-1:500
Gene Alias:
glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, NMDA receptor subtype 2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, subunit epsilon-1
Immunogen:
Amino acid region 1099-1213 from human NMDAR2A produced as a GST fusion protein.
Quantity:
0.025 mg
Primary or Secondary:
Primary
Target Species:
Rat, Human
Form:
__