Classification:
Polyclonal
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
Purification Method:
Affinity Purified
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus species, Zebrafish
Applications:
ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Gel Shift, ChIP Assay, Immunoassay
Dilution:
Western Blot 1:500 - 1:1000, Simple Western 1:100, ELISA, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Immunoblotting, Gel Super Shift Assays 1:1 - 1:100, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635), Dual RNAscope ISH-IHC
Gene Accession No.:
Q61221, O35800
Immunogen:
This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].
Research Discipline:
Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.