Classification:
Monoclonal
Gene Alias:
conserved helix-loop-helix ubiquitous kinaseIKBKA, EC 2.7.11, EC 2.7.11.10, IkappaB kinase, I-kappa-B kinase 1, I-kappa-B kinase alpha, IkBKA, IKK1I-kappa-B kinase-alpha, IKK-A, IKKAIkB kinase alpha subunit, IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKAIKK-a kinase, Nuclear factor NFkappaB inhibitor kinase alpha, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16, TCF-16, Transcription factor 16
Purification Method:
Protein G purified
Primary or Secondary:
Primary
Test Specificity:
An 85 kDa band should be observed.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Applications:
Western Blot, ChIP Assay, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Dilution:
Western Blot 1 ug/ml, Simple Western 10 ug/ml, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 27705798), Flow Cytometry (Intracellular): 0.25-0.5 ug/10^6 cells, Immunohistochemistry 1:200. Use reported in scientific literature (PMID 27121058), Immunocytochemistry/Immunofluorescence 1:10, Immunoprecipitation 1-2 ug/ml, Immunohistochemistry-Paraffin 5ug/ml, Immunohistochemistry-Frozen reported in scientific literature (PMID 25133425), Flow (Intracellular) Flow Cytometry: (Intracellular): 0.25-0.5 ug/10^6 cells. Use reported in scientific literature (PMID 24804954), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Gene Accession No.:
O15111
Immunogen:
This antibody was raised against a His-tagged full-length human IKK alpha protein.
Research Discipline:
Cancer, DNA Repair, DNA replication Transcription Translation and Splicing, Immunology, Innate Immunity, mTOR Pathway, Protein Kinase, Signal Transduction
Target Species:
Human, Mouse, Rat, Primate