NB11074679

HIF-1 alpha, Hydroxy Pro564 Antibody, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NB11074679-Each-of-1 In Stock ₹ 58,351.92

NB11074679 - Each of 1

₹ 58,351.92

In Stock

Quantity

1

Base Price: ₹ 58,351.92

GST (18%): ₹ 10,503.346

Total Price: ₹ 68,855.266

Antigen

HIF-1 alpha (Hydroxy Pro564)

Classification

Polyclonal

Dilution

Western Blot 1:500-1:2000, ELISA 1:5000-1:20000, Immunohistochemistry 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100-1:2000

Gene Accession No.

Q16665

Gene Symbols

HIF1A

Immunogen

This HIF-1 alpha [Hydroxy Pro564] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1 alpha. (Uniprot: Q16665)

Purification Method

Unpurified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus species sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Content And Storage

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Applications

Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoblot

Conjugate

Unconjugated

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Alias

AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp

Host Species

Rabbit

Molecular Weight of Antigen

93 kDa

Quantity

0.1 mL

Research Discipline

Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators

Gene ID (Entrez)

3091

Target Species

Human, Mouse, Rat, Bovine, Canine, Monkey, Xenopus species

Form

Antisera

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Description

  • HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse samples
  • It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoblotting.

Compare Similar Items

Show Difference

Img

Novus Biologicals

NB11074679

--


Antigen:
HIF-1 alpha (Hydroxy Pro564)

Classification:
Polyclonal

Dilution:
Western Blot 1:500-1:2000, ELISA 1:5000-1:20000, Immunohistochemistry 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100-1:2000

Gene Accession No.:
Q16665

Gene Symbols:
HIF1A

Immunogen:
This HIF-1 alpha [Hydroxy Pro564] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1 alpha. (Uniprot: Q16665)

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus species sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoblot

Conjugate:
Unconjugated

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Alias:
AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp

Host Species:
Rabbit

Molecular Weight of Antigen:
93 kDa

Quantity:
0.1 mL

Research Discipline:
Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators

Gene ID (Entrez):
3091

Target Species:
Human, Mouse, Rat, Bovine, Canine, Monkey, Xenopus species

Form:
Antisera

Img

Fischer Scientific

NB11074682

--


Antigen:
Aquaporin-2

Classification:
Polyclonal

Dilution:
Western Blot 1 - 2 ug/ml, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence 1 ug/ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen reported in scientific literature

Gene Accession No.:
P34080

Gene Symbols:
AQP2

Immunogen:
Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Human, rat and mouse. 100% sequence identity with sheep, porcine, and bovine proteins.

Content And Storage:
Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% Sodium Azide

Gene Alias:
ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
Plasma Membrane Markers, Signal Transduction

Gene ID (Entrez):
359

Target Species:
Human, Mouse, Rat

Form:
__

Img

Fischer Scientific

NB11074682T

--


Antigen:
Aquaporin-2

Classification:
Polyclonal

Dilution:
Western Blot 1 - 2 ug/ml, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence 1 ug/ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen reported in scientific literature

Gene Accession No.:
P34080

Gene Symbols:
AQP2

Immunogen:
Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Human, rat and mouse. 100% sequence identity with sheep, porcine, and bovine proteins.

Content And Storage:
Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% Sodium Azide

Gene Alias:
ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.025 mL

Research Discipline:
Plasma Membrane Markers, Signal Transduction

Gene ID (Entrez):
359

Target Species:
Human, Mouse, Rat

Form:
__

Img

Fischer Scientific

NB11074730

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Antigen:
Opsin 1 (Medium Wave)

Classification:
Polyclonal

Dilution:
Western Blot 1:250-1:500, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen 1:250-1:500

Gene Accession No.:
O35599

Gene Symbols:
OPN1MW

Immunogen:
A synthetic peptide from mouse Opsin 1 (Medium Wave) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
__

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Formulation:
Lyophilized from whole antisera with No Preservative

Gene Alias:
GCP, GOP, Green cone photoreceptor pigment, Green-sensitive opsin, medium-wave-sensitive opsin 1, OPN1MW, opsin 1 (cone pigments), medium-wave-sensitive 2

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
__

Gene ID (Entrez):
2652

Target Species:
Mouse, Rat

Form:
Antisera