Classification:
Polyclonal
Concentration:
1.00 mg/mL
Dilution:
Western Blot 2 ug/mL. Use reported in scientific literature (PMID 31082627), Simple Western 1:50, Flow Cytometry reported in scientific literature (PMID 21422470), Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200. Use reported in multiple pieces of scientific literature (PMID 23980916), Immunohistochemistry-Paraffin 1:400. Use reported in scientific literature (PMID 31022918), Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916), In-situ Hybridization reported in scientific literature (PMID 27133471), Flow (Cell Surface) 1:10 - 1:1000, Single Cell Western 1:10, Dual RNAscope ISH-IHC
Gene Alias:
antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, Cell surface glycoprotein MAC-1 subunit alpha, Complement component 3 receptor 3 subunit, CR-3 alpha chain, CR3A, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement
Purification Method:
Affinity Purified
Primary or Secondary:
Primary
Test Specificity:
Mouse, rat and human. We have feedback that this antibody does not work in human samples with Western blot. Immunogen has 100% homology to bovine and 88% homology with sheep.
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), In Situ Hybridization (ISH), Flow Cytometry, In Situ Hybridization (ISH)
Formulation:
PBS with 0.02% Sodium Azide
Immunogen:
Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]
Research Discipline:
Breast Cancer, Cancer, Cellular Markers, Cytoskeleton Markers, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Microglia Markers, Myeloid Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Target Species:
Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque