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NB200598Z

RNA Polymerase II/POLR2A Antibody (4H8), Alexa Fluor™ 700, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NB200598Z-Each-of-1 In Stock ₹ 54,245.04

NB200598Z - Each of 1

₹ 54,245.04

In Stock

Quantity

1

Base Price: ₹ 54,245.04

GST (18%): ₹ 9,764.107

Total Price: ₹ 64,009.147

Antigen

RNA Polymerase II/POLR2A

Classification

Monoclonal

Conjugate

Alexa Fluor 700

Gene Accession No.

P24928

Gene Symbols

POLR2A

Immunogen

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn

Content And Storage

Store at 4C in the dark.

Isotype

IgG1

Applications

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence

Clone

4H8

Dilution

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)

Gene Alias

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species

Mouse

Molecular Weight of Antigen

217 kDa

Quantity

0.1 mL

Research Discipline

Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators

Gene ID (Entrez)

5430

Target Species

Human, Mouse, Yeast, Primate (Negative)

Form

Purified

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Description

  • RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular).

Compare Similar Items

Show Difference

Img

Novus Biologicals

NB200598Z

--

Antigen:
RNA Polymerase II/POLR2A
Classification:
Monoclonal
Conjugate:
Alexa Fluor 700
Gene Accession No.:
P24928
Gene Symbols:
POLR2A
Immunogen:
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG1
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone:
4H8
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
Gene Alias:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Host Species:
Mouse
Molecular Weight of Antigen:
217 kDa
Quantity:
0.1 mL
Research Discipline:
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators
Gene ID (Entrez):
5430
Target Species:
Human, Mouse, Yeast, Primate (Negative)
Form:
Purified

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Novus Biologicals

NB200599

--

Antigen:
Rift Valley Fever Virus Nucleocapsid
Classification:
Polyclonal
Conjugate:
DyLight 650
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
Antibody was raised against a 17 amino acid peptide near the center of the RVF virus nucleocapsid. The immunogen is located within amino acids 70 - 120 of Rift Valley Fever Virus Nucleocapsid. Amino Acid Squence: LINKYKLKEGNPSRDEL
Purification Method:
Peptide affinity purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG
Applications:
ELISA
Clone:
__
Dilution:
__
Gene Alias:
Fragment, NP, nucleocapsid, Nucleocapsid protein, Nucleocapsid proteinNucleoprotein, Nucleoprotein, RVFV_sS_gp2
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
0.1 mL
Research Discipline:
Infections (Virus Bacteria and Parasites)
Gene ID (Entrez):
9538291
Target Species:
Virus
Form:
Purified

Img

Novus Biologicals

NB200601

--

Antigen:
Seasonal H1N1 Hemagglutinin
Classification:
Polyclonal
Conjugate:
Alexa Fluor 405
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
Antibody was raised against a synthetic peptide from the Hemagglutinin protein. The peptide sequence is unique from the peptide sequence for product NBP2-41104, and NBP2-41106. This antibody is a cognate pair with product number NBP2-41109. The immunogen is located within amino acids 150 - 200 of Seasonal H1N1 Hemagglutinin. Amino Acid Squence: TGKNGLYPN
Purification Method:
Peptide affinity purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG
Applications:
ELISA
Clone:
__
Dilution:
__
Gene Alias:
Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
0.1 mL
Research Discipline:
Infections (Virus Bacteria and Parasites)
Gene ID (Entrez):
__
Target Species:
Influenza virus N1, Virus
Form:
Purified

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Novus Biologicals

NB200602

--

Antigen:
TSPY1
Classification:
Polyclonal
Conjugate:
Alexa Fluor 405
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
Antibody was raised against a 20 amino acid peptide near the carboxy terminus of human TSPY1L. The immunogen is located within the last 50 amino acids of TSPY1L. Amino Acid Squence: CRNPLQYYKRMKPPEEGTETS
Purification Method:
Peptide affinity purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4°C in the dark.
Isotype:
IgG
Applications:
Western Blot, ELISA
Clone:
__
Dilution:
__
Gene Alias:
Cancer/testis antigen 78, CT78DYS14, testis specific protein, Y-linked, testis specific protein, Y-linked 1, testis-specific Y-encoded protein 1, TSPYpJA923
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
0.1 mL
Research Discipline:
Cancer, Cell Cycle and Replication
Gene ID (Entrez):
7258
Target Species:
Human, Mouse
Form:
Purified