Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
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Antigen
Biotin (Vitamin B7 or Vitamin H)
Classification
Monoclonal
Concentration
1 mg/mL
Dilution
Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Host Species
Mouse
Molecular Weight of Antigen
2.4 kDa
Quantity
200 μg
Test Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG1
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Clone
BTN/2032R
Conjugate
Unconjugated
Formulation
10mM PBS - BSA free with No Preservative
Immunogen
Biotinylated sheep immunoglobulin
Purification Method
Protein A or G purified
Primary or Secondary
Primary
Target Species
All species
Form
Purified
Related Products
Description
- Biotin (Vitamin B7 or Vitamin H) Monoclonal specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization, CyTOF-ready.
Compare Similar Items
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Antigen: Biotin (Vitamin B7 or Vitamin H)
Classification: Monoclonal
Concentration: 1 mg/mL
Dilution: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Host Species: Mouse
Molecular Weight of Antigen: 2.4 kDa
Quantity: 200 μg
Test Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Clone: BTN/2032R
Conjugate: Unconjugated
Formulation: 10mM PBS - BSA free with No Preservative
Immunogen: Biotinylated sheep immunoglobulin
Purification Method: Protein A or G purified
Primary or Secondary: Primary
Target Species: All species
Form: Purified
Antigen: mpp8
Classification: Polyclonal
Concentration: __
Dilution: Western Blot
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 0.1 mL
Test Specificity: __
Content And Storage: Store at 4°C in the dark.
Isotype: IgG
Applications: Western Blot
Clone: __
Conjugate: Janelia Fluor 549
Formulation: 50mM Sodium Borate
Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human M-phase Phosphoprotein 8 using the numbering given in entry NP_059990.2 (GeneID 54737).
Purification Method: Immunogen affinity purified
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen: MLK4
Classification: Polyclonal
Concentration: __
Dilution: Western Blot
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 0.1 mL
Test Specificity: __
Content And Storage: Store at 4°C in the dark.
Isotype: IgG
Applications: Western Blot
Clone: __
Conjugate: Alexa Fluor 647
Formulation: 50mM Sodium Borate
Immunogen: The immunogen for this product maps to a region between residue 650 and 700 of human mixed lineage kinase 4 using the numbering given in entry NP_115811.2 (GeneID 84451).
Purification Method: Immunogen affinity purified
Primary or Secondary: Primary
Target Species: Human
Form: Purified
