NB300251

Notch-1 Antibody - (Cleaved N terminal), Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NB300251-Each-of-1 In Stock ₹ 60,448.14

NB300251 - Each of 1

₹ 60,448.14

In Stock

Quantity

1

Base Price: ₹ 60,448.14

GST (18%): ₹ 10,880.665

Total Price: ₹ 71,328.805

Antigen

Notch-1

Classification

Polyclonal

Dilution

Western Blot 1:500-1:2000, ELISA 1:20000-1:60000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:200

Gene Accession No.

P46531

Gene Symbols

NOTCH1

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). (Uniprot: P46531)

Purification Method

Unpurified

Regulatory Status

RUO

Gene ID (Entrez)

4851

Target Species

Human, Mouse, Rat

Form

Antisera

Applications

Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.1% Sodium Azide

Gene Alias

EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1

Host Species

Rabbit

Molecular Weight of Antigen

272.5 kDa

Quantity

0.2 mL

Primary or Secondary

Primary

Test Specificity

This antiserum is directed against human Notch-1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat Notch-1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Content And Storage

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

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Description

  • Notch-1 Polyclonal specifically detects Notch-1 in Human, Mouse samples
  • It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Compare Similar Items

Show Difference

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Novus Biologicals

NB300251

--


Antigen:
Notch-1

Classification:
Polyclonal

Dilution:
Western Blot 1:500-1:2000, ELISA 1:20000-1:60000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:200

Gene Accession No.:
P46531

Gene Symbols:
NOTCH1

Immunogen:
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). (Uniprot: P46531)

Purification Method:
Unpurified

Regulatory Status:
RUO

Gene ID (Entrez):
4851

Target Species:
Human, Mouse, Rat

Form:
Antisera

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.1% Sodium Azide

Gene Alias:
EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1

Host Species:
Rabbit

Molecular Weight of Antigen:
272.5 kDa

Quantity:
0.2 mL

Primary or Secondary:
Primary

Test Specificity:
This antiserum is directed against human Notch-1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat Notch-1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Img

Novus Biologicals

NB30025101

--


Antigen:
Notch-1

Classification:
Polyclonal

Dilution:
Western Blot 1:500-1:2000, ELISA 1:20000-1:60000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Dot Blot 1:100-1:2000

Gene Accession No.:
P46531

Gene Symbols:
NOTCH1

Immunogen:
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).

Purification Method:
__

Regulatory Status:
__

Gene ID (Entrez):
4851

Target Species:
Human, Mouse, Rat

Form:
__

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Dot Blot

Conjugate:
Unconjugated

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.1% sodium azide

Gene Alias:
EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Notch-1 does not react with mouse N2, N3, or N4. It only recognizes the cleaved intracellular (activated) form (NICD).

Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.

Img

Novus Biologicals™

NB300251PEP

--


Antigen:
__

Classification:
__

Dilution:
__

Gene Accession No.:
__

Gene Symbols:
__

Immunogen:
__

Purification Method:
__

Regulatory Status:
RUO - research use only

Gene ID (Entrez):
4851

Target Species:
__

Form:
__

Applications:
__

Conjugate:
__

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Gene Alias:
EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1 Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1

Host Species:
__

Molecular Weight of Antigen:
__

Quantity:
0.05 mg

Primary or Secondary:
__

Test Specificity:
__

Content And Storage:
Store at -20 C short term. Aliquot and store (at -80 C long term. Avoid freeze-thaw cycles.)

Img

Novus Biologicals

NB300254

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Antigen:
SLC6A3/DAT1

Classification:
Polyclonal

Dilution:
Western Blot 1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:1000

Gene Accession No.:
Q01959

Gene Symbols:
SLC6A3

Immunogen:
Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH. Accession # Q01959

Purification Method:
Antigen Affinity-purified

Regulatory Status:
RUO

Gene ID (Entrez):
6531

Target Species:
Human, Mouse, Hamster, Primate, Golden Syrian Hamster

Form:
__

Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Formulation:
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol with No Preservative

Gene Alias:
DA transporter, DATDAT1Solute carrier family 6 member 3, sodium-dependent dopamine transporter, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3

Host Species:
Rabbit

Molecular Weight of Antigen:
80 kDa

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Specific for the ∼88k DAT protein in Western blots.

Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.