Antigen:
ERK1 (p Thr202, p Tyr204)
Classification:
Polyclonal
Formulation:
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol with No Preservative
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding ERK1 conjugated to KLH. Accession # P63086
Purification Method:
Antigen Affinity-purified
Primary or Secondary:
Primary
Test Specificity:
Specific for the ∼42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.
Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Dilution:
Western Blot 1:1000, Immunohistochemistry 1:500, Immunohistochemistry-Paraffin
Gene Alias:
EC 2.7.11, ERK-1, ERK1p44-MAPK, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MGC20180, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, PRKM3EC 2.7.11.24
Molecular Weight of Antigen:
42 kDa
Research Discipline:
Angiogenesis, Breast Cancer, Cancer, Cell Cycle and Replication, Diabetes Research, Hypoxia, MAP Kinase Signaling, Phospho Specific, Signal Transduction
Target Species:
Human, Mouse, Rat, Chicken