IL-1 beta/IL-1F2 Antibody, Novus Biologicals™
Manufacturer: Novus Biologicals
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB600633-Each-of-1 | In Stock | ₹ 36,576.90 |
NB600633 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 36,576.90
GST (18%): ₹ 6,583.842
Total Price: ₹ 43,160.742
Antigen
IL-1 beta/IL-1F2
Classification
Polyclonal
Conjugate
Unconjugated
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with No Preservative
Gene Alias
catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Host Species
Rabbit
Molecular Weight of Antigen
30.75 kDa
Quantity
0.1 mg
Research Discipline
Adaptive Immunity, Apoptosis, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity, Metastasis
Gene ID (Entrez)
3553
Target Species
Human, Mouse, Rat, Porcine, Canine, Monkey, Golden Syrian Hamster
Form
Purified
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Dot Blot, Immunoassay, Immunohistochemistry
Concentration
2 mg/ml
Dilution
Western Blot 1:1000, Flow Cytometry, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen, Dot Blot, Electron Microscopy 1:10-1:500, Immunohistochemistry Whole-Mount
Gene Accession No.
P01584
Gene Symbols
IL1B
Immunogen
This IL-1 beta/IL-1F2 Antibody was prepared by repeated immunizations with recombinant human IL-1 beta/IL-1F2 produced in E.coli. The MW of the recombinant 153 aa IL-1 beta/IL-1F2 was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta/IL-1F2 converting enzyme (ICE, capase-1). (Uniprot: P01584)
Purification Method
Whole Antiserum
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody is primarily directed against mature, 17,000 MW human IL-1 beta/IL-1F2and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1 beta/IL-1F2in the same formats using similar dilutions. The antiserum does not recognize human IL-1alpha. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta/IL-1F2containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1beta precursor molecule. Denatured 31,000 precursor IL-1 beta/IL-1F2will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta/IL-1F2exposing epitopes not exposed in the nat
Content And Storage
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype
IgG
Related Products
Description
- IL-1 beta/IL-1F2 Polyclonal antibody specifically detects IL-1 beta/IL-1F2 in Human, Mouse, Rat, Porcine, Canine, Monkey, Golden Syrian Hamster samples
- It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoassay, Immunohistochemistry Whole-Mount.
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Antigen: IL-1 beta/IL-1F2
Classification: Polyclonal
Conjugate: Unconjugated
Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with No Preservative
Gene Alias: catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Host Species: Rabbit
Molecular Weight of Antigen: 30.75 kDa
Quantity: 0.1 mg
Research Discipline: Adaptive Immunity, Apoptosis, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity, Metastasis
Gene ID (Entrez): 3553
Target Species: Human, Mouse, Rat, Porcine, Canine, Monkey, Golden Syrian Hamster
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Dot Blot, Immunoassay, Immunohistochemistry
Concentration: 2 mg/ml
Dilution: Western Blot 1:1000, Flow Cytometry, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen, Dot Blot, Electron Microscopy 1:10-1:500, Immunohistochemistry Whole-Mount
Gene Accession No.: P01584
Gene Symbols: IL1B
Immunogen: This IL-1 beta/IL-1F2 Antibody was prepared by repeated immunizations with recombinant human IL-1 beta/IL-1F2 produced in E.coli. The MW of the recombinant 153 aa IL-1 beta/IL-1F2 was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta/IL-1F2 converting enzyme (ICE, capase-1). (Uniprot: P01584)
Purification Method: Whole Antiserum
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody is primarily directed against mature, 17,000 MW human IL-1 beta/IL-1F2and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1 beta/IL-1F2in the same formats using similar dilutions. The antiserum does not recognize human IL-1alpha. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta/IL-1F2containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1beta precursor molecule. Denatured 31,000 precursor IL-1 beta/IL-1F2will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta/IL-1F2exposing epitopes not exposed in the nat
Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype: IgG
Antigen: hCG beta 4
Classification: Monoclonal
Conjugate: Alexa Fluor 405
Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
Gene Alias: CGB3choriogonadotropin subunit beta, CGB5, CGB7, CGB8, CG-beta, Chorionic gonadotrophin chain beta, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit, chorionic gonadotropin, beta polypeptide, hCGB
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 0.25 mL
Research Discipline: Cancer
Gene ID (Entrez): 1082
Target Species: Human
Form: Purified
Applications: ELISA
Concentration: __
Dilution: ELISA
Gene Accession No.: __
Gene Symbols: CGB3
Immunogen: Human CG.
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This is a high affinity antibody recognising the beta 4 epitope on hCG. It is also reactive with hCGbeta, hCGbetacf (fragment), hCGn (nicked), hCGbetan, CTP-hCG, CTP-hCGbeta. It may be used in combination with INN hCG2, 32 and 45. It has minor cross reactivity with hLH and hLHbeta. No cross reaction is observed with hFSH, hTSH, or the glycoprotein hormone alpha chains.
Content And Storage: Store at 4C in the dark.
Isotype: IgG1
Antigen: hCG beta 4
Classification: Monoclonal
Conjugate: DyLight 488
Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
Gene Alias: CGB3choriogonadotropin subunit beta, CGB5, CGB7, CGB8, CG-beta, Chorionic gonadotrophin chain beta, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit, chorionic gonadotropin, beta polypeptide, hCGB
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 0.25 mL
Research Discipline: Cancer
Gene ID (Entrez): 1082
Target Species: Human
Form: Purified
Applications: ELISA
Concentration: __
Dilution: ELISA
Gene Accession No.: __
Gene Symbols: CGB3
Immunogen: Human CG.
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This is a high affinity antibody recognising the beta 4 epitope on hCG. It is also reactive with hCGbeta, hCGbetacf (fragment), hCGn (nicked), hCGbetan, CTP-hCG, CTP-hCGbeta. It may be used in combination with INN hCG2, 32 and 45. It has minor cross reactivity with hLH and hLHbeta. No cross reaction is observed with hFSH, hTSH, or the glycoprotein hormone alpha chains.
Content And Storage: Store at 4C in the dark.
Isotype: IgG1