Classification:
Polyclonal
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Immunogen:
Collagen II from human knee cartilage and bovine nasal cartilage. (Uniprot: P02458)
Purification Method:
Affinity Purified
Target Species:
Human, Mouse, Rat, Bovine, Sheep, Canine, Silkworm
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Dilution:
Western Blot 1:1000-1:10000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:400, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen
Gene Accession No.:
P02458
Molecular Weight of Antigen:
141.79 kDa
Primary or Secondary:
Primary
Test Specificity:
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Content And Storage:
Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.