NBP197410

DYKDDDDK Epitope Tag Antibody (29E4.G7), Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP197410-Each-of-1 In Stock ₹ 72,212.64

NBP197410 - Each of 1

₹ 72,212.64

In Stock

Quantity

1

Base Price: ₹ 72,212.64

GST (18%): ₹ 12,998.275

Total Price: ₹ 85,210.915

Antigen

DYKDDDDK Epitope Tag

Classification

Monoclonal

Concentration

1.0 mg/mL

Dilution

Western Blot 1:2000-1:10000, Simple Western, Flow Cytometry, ELISA 1:150000-1:250000, Immunohistochemistry 1:1000-1:5000

Host Species

Mouse

Molecular Weight of Antigen

1.01 kDa

Quantity

0.5 mg

Primary or Secondary

Primary

Content And Storage

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Target Molecule

Epitope Tag

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

Clone

29E4.G7

Conjugate

Unconjugated

Gene Alias

DDDDK epitope tag, DDK, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, epitope tag flag, FLAG (FLAG is a trademark of Sigma Aldrich), Flag tag, Ty 1 Tag, xxxDDDDK epitope tag

Immunogen

This DYKDDDDK Epitope Tag Antibody (29E4.G7) was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG(TM) epitope tag peptide DYKDDDDK Epitope Tag Epitope Tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.

Purification Method

Affinity Purified

Regulatory Status

RUO

Test Specificity

The purified antibody is directed against the FLAG(TM) motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG(TM) tag antibody detects over-expressed proteins containing the FLAG(TM) epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Isotype

IgG2a κ

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Description

  • DYKDDDDK Epitope Tag Monoclonal antibody specifically detects DYKDDDDK Epitope Tag in Epitope Tag samples
  • It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry.

Compare Similar Items

Show Difference

Img

Novus Biologicals

NBP197410

--


Antigen:
DYKDDDDK Epitope Tag

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Western Blot 1:2000-1:10000, Simple Western, Flow Cytometry, ELISA 1:150000-1:250000, Immunohistochemistry 1:1000-1:5000

Host Species:
Mouse

Molecular Weight of Antigen:
1.01 kDa

Quantity:
0.5 mg

Primary or Secondary:
Primary

Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Target Molecule:
Epitope Tag

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

Clone:
29E4.G7

Conjugate:
Unconjugated

Gene Alias:
DDDDK epitope tag, DDK, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, epitope tag flag, FLAG (FLAG is a trademark of Sigma Aldrich), Flag tag, Ty 1 Tag, xxxDDDDK epitope tag

Immunogen:
This DYKDDDDK Epitope Tag Antibody (29E4.G7) was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG(TM) epitope tag peptide DYKDDDDK Epitope Tag Epitope Tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
The purified antibody is directed against the FLAG(TM) motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG(TM) tag antibody detects over-expressed proteins containing the FLAG(TM) epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Isotype:
IgG2a κ

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Fischer Scientific

NBP197416

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Antigen:
GABARAP

Classification:
Polyclonal

Concentration:
1.12 mg/mL

Dilution:
Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Western Blot (Negative), ICC/IF (Negative)

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Target Molecule:
__

Applications:
Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry

Clone:
__

Conjugate:
Unconjugated

Gene Alias:
ATG8A, FLC3B, FLJ25768, GABA(A) receptor-associated proteinMGC120154, gamma-aminobutyric acid receptor-associated protein, MM46MGC120155

Immunogen:
A synthetic peptide made to an N-terminal region of the human GABARAP protein (within residues 1-50). [Swiss-Prot O95166]

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
__

Isotype:
IgG

Img

Novus Biologicals

NBP197416B

--


Antigen:
GABARAP

Classification:
Polyclonal

Concentration:
__

Dilution:
Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Western Blot (Negative), ICC/IF (Negative)

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Primary or Secondary:
Primary

Content And Storage:
Store at 4C in the dark.

Target Molecule:
__

Applications:
Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry

Clone:
__

Conjugate:
Biotin

Gene Alias:
ATG8A, FLC3B, FLJ25768, GABA(A) receptor-associated proteinMGC120154, gamma-aminobutyric acid receptor-associated protein, MM46MGC120155

Immunogen:
A synthetic peptide made to an N-terminal region of the human GABARAP protein (within residues 1-50). [Swiss-Prot O95166]

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
__

Isotype:
IgG

Img

Novus Biologicals

NBP197416F

--


Antigen:
GABARAP

Classification:
Polyclonal

Concentration:
__

Dilution:
Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Western Blot (Negative), ICC/IF (Negative)

Host Species:
Rabbit

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Primary or Secondary:
Primary

Content And Storage:
Store at 4C in the dark.

Target Molecule:
__

Applications:
Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry

Clone:
__

Conjugate:
FITC

Gene Alias:
ATG8A, FLC3B, FLJ25768, GABA(A) receptor-associated proteinMGC120154, gamma-aminobutyric acid receptor-associated protein, MM46MGC120155

Immunogen:
A synthetic peptide made to an N-terminal region of the human GABARAP protein (within residues 1-50). [Swiss-Prot O95166]

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
__

Isotype:
IgG