Classification:
Monoclonal
Purification Method:
Ammonium sulfate precipitation
Target Species:
Human, Rabbit
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoassay
Dilution:
Western Blot 1:500-1:2000, ELISA 1:10000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:100. Use reported by customer review, Immunohistochemistry-Paraffin 1:200-1:1000, Immunohistochemistry-Frozen reported in scientific literature (PMID 33131911), Knockout Validated
Gene Alias:
antigen identified by monoclonal Ki-67, antigen KI-67, Antigen Ki67, Ki67, Ki-67, KIA, Marker Of Proliferation Ki-67, MIB-, MIB-1, PPP1R105, Proliferation Marker Protein Ki-67, proliferation-related Ki-67 antigen, Protein Phosphatase 1, Regulatory Subunit 105
Immunogen:
The immunogen for this KI67/MKI67 Antibody (8D5) was made using a synthetic peptide from the internal region of Human KI67/MKI67 (amino acids: 1100-1113, 1222-1235 and 3 more spots); sequence: CEDLAGFKELFQTPG [Uniprot: P46013].
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.