NBP233074UV

p53 Antibody (BP53-12), DyLight 350, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP233074UV-Each-of-1 In Stock ₹ 56,298.48

NBP233074UV - Each of 1

₹ 56,298.48

In Stock

Quantity

1

Base Price: ₹ 56,298.48

GST (18%): ₹ 10,133.726

Total Price: ₹ 66,432.206

Antigen

p53

Classification

Monoclonal

Conjugate

DyLight 350

Gene Alias

Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53

Host Species

Mouse

Molecular Weight of Antigen

53 kDa

Quantity

0.1 mL

Research Discipline

Apoptosis, Cancer, Cell Cycle and Replication, Cellular Markers, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, HIF Target Genes, Hypoxia, Neuroscience, Neurotransmission, p53 Pathway, Phospho Specific, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors

Gene ID (Entrez)

7157

Target Species

Human, Mouse, Canine, Chicken, Hamster, Monkey, Mouse (Negative), Rat (Negative)

Form

Purified

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone

BP53-12

Dilution

Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500

Gene Symbols

TP53

Immunogen

Recombinant human wild-type p53 protein (Uniprot: P04637)

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This monoclonal antibody reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53

Content And Storage

Store at 4C in the dark.

Isotype

IgG2a κ

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Description

  • Description p53 Monoclonal specifically detects p53 in Human, Canine, Chicken, Hamster, Monkey, Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Compare Similar Items

Show Difference

Img

Novus Biologicals

NBP233074UV

--


Antigen:
p53

Classification:
Monoclonal

Conjugate:
DyLight 350

Gene Alias:
Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53

Host Species:
Mouse

Molecular Weight of Antigen:
53 kDa

Quantity:
0.1 mL

Research Discipline:
Apoptosis, Cancer, Cell Cycle and Replication, Cellular Markers, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, HIF Target Genes, Hypoxia, Neuroscience, Neurotransmission, p53 Pathway, Phospho Specific, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors

Gene ID (Entrez):
7157

Target Species:
Human, Mouse, Canine, Chicken, Hamster, Monkey, Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
BP53-12

Dilution:
Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500

Gene Symbols:
TP53

Immunogen:
Recombinant human wild-type p53 protein (Uniprot: P04637)

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This monoclonal antibody reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG2a κ

Img

Novus Biologicals

NBP233076

--


Antigen:
CD34

Classification:
Monoclonal

Conjugate:
Unconjugated

Gene Alias:
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mg

Research Discipline:
Adaptive Immunity, Angiogenesis, B Cell Development and Differentiation Markers, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors

Gene ID (Entrez):
947

Target Species:
Human, Rat

Form:
Purified

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
ICO-115

Dilution:
Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready

Gene Symbols:
CD34

Immunogen:
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for the CD34 antibody.

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but shows low specificity.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Img

Novus Biologicals

NBP233076B

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Antigen:
CD34

Classification:
Monoclonal

Conjugate:
Biotin

Gene Alias:
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
Adaptive Immunity, Angiogenesis, B Cell Development and Differentiation Markers, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors

Gene ID (Entrez):
947

Target Species:
Human, Rat

Form:
Purified

Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
ICO-115

Dilution:
Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Symbols:
CD34

Immunogen:
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for the CD34 antibody.

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but shows low specificity.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Img

Novus Biologicals

NBP233076G

--


Antigen:
CD34

Classification:
Monoclonal

Conjugate:
DyLight 488

Gene Alias:
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
Adaptive Immunity, Angiogenesis, B Cell Development and Differentiation Markers, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors

Gene ID (Entrez):
947

Target Species:
Human, Rat

Form:
Purified

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
ICO-115

Dilution:
Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Symbols:
CD34

Immunogen:
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for the CD34 antibody.

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but shows low specificity.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ