NBP234395Z

Cytokeratin, Basic (Type II or HMW) Antibody (SPM116), Alexa Fluor™ 700, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP234395Z-Each-of-1 In Stock ₹ 55,271.76

NBP234395Z - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

Cytokeratin, Basic (Type II or HMW)

Classification

Monoclonal

Conjugate

Alexa Fluor 700

Gene Alias

CK1, CK10, CK14, CK5, cytokeratin 1, cytokeratin 10, cytokeratin 14, cytokeratin 5

Immunogen

Human epidermal keratin (Uniprot: Q01546)

Purification Method

Protein A or G purified

Regulatory Status

RUO

Test Specificity

This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Content And Storage

Store at 4C in the dark.

Isotype

IgG1 κ

Applications

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone

SPM116

Dilution

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Host Species

Mouse

Molecular Weight of Antigen

59 kDa

Quantity

0.1 mL

Primary or Secondary

Primary

Target Species

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit

Form

Purified

Description

  • Cytokeratin, Basic (Type II or HMW) Monoclonal specifically detects Cytokeratin, Basic (Type II or HMW) in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Novus Biologicals

NBP234395Z

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Antigen:
Cytokeratin, Basic (Type II or HMW)

Classification:
Monoclonal

Conjugate:
Alexa Fluor 700

Gene Alias:
CK1, CK10, CK14, CK5, cytokeratin 1, cytokeratin 10, cytokeratin 14, cytokeratin 5

Immunogen:
Human epidermal keratin (Uniprot: Q01546)

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
SPM116

Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Host Species:
Mouse

Molecular Weight of Antigen:
59 kDa

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit

Form:
Purified

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Novus Biologicals

NBP234396B

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Antigen:
Kappa Light Chain

Classification:
Monoclonal

Conjugate:
Biotin

Gene Alias:
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Immunogen:
Human B-Lymphoma Cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Applications:
Western Blot, ELISA, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
SPM508

Dilution:
Western Blot, ELISA, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Host Species:
Mouse

Molecular Weight of Antigen:
22.5 kDa

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human, Rat (Negative)

Form:
Purified

Img

Novus Biologicals

NBP234396H

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Antigen:
Kappa Light Chain

Classification:
Monoclonal

Conjugate:
HRP

Gene Alias:
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Immunogen:
Human B-Lymphoma Cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Applications:
Western Blot, ELISA, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
SPM508

Dilution:
Western Blot, ELISA, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Host Species:
Mouse

Molecular Weight of Antigen:
22.5 kDa

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human, Rat (Negative)

Form:
Purified

Img

Novus Biologicals

NBP234396P

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Antigen:
Kappa Light Chain

Classification:
Monoclonal

Conjugate:
PerCP

Gene Alias:
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Immunogen:
Human B-Lymphoma Cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
SPM508

Dilution:
Flow Cytometry

Host Species:
Mouse

Molecular Weight of Antigen:
22.5 kDa

Quantity:
0.1 mL

Primary or Secondary:
Primary

Target Species:
Human, Rat (Negative)

Form:
Purified