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NBP234770Z

Tyrosinase Antibody (SPM360), Alexa Fluor™ 700, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NBP234770Z-Each-of-1 In Stock ₹ 55,271.76

NBP234770Z - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

Tyrosinase

Classification

Monoclonal

Conjugate

Alexa Fluor 700

Gene Alias

CMM8, EC 1.14.18.1, LB24-AB, Monophenol monooxygenase, OCA1A, OCAIA, SHEP3, SK29-AB, Tumor rejection antigen AB, tyrosinase, tyrosinase (oculocutaneous albinism IA)

Host Species

Mouse

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This monoclonal antibody shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this monoclonal antibody shows tyrosinase in melanotic as well as amelanotic variants. This monoclonal antibody is a useful marker for melanocytes and melanomas.

Content And Storage

Store at 4C in the dark.

Isotype

IgG2a κ

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone

SPM360

Dilution

Western Blot, Flow Cytometry, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Symbols

TYR

Immunogen

Recombinant tyrosinase protein (Uniprot: P14679 )

Quantity

0.1 mL

Research Discipline

Lipid and Metabolism

Gene ID (Entrez)

7299

Target Species

Human

Form

Purified

Description

  • Tyrosinase Monoclonal specifically detects Tyrosinase in Human samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Compare Similar Items

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Img

Novus Biologicals

NBP234770Z

--

Antigen:
Tyrosinase
Classification:
Monoclonal
Conjugate:
Alexa Fluor 700
Gene Alias:
CMM8, EC 1.14.18.1, LB24-AB, Monophenol monooxygenase, OCA1A, OCAIA, SHEP3, SK29-AB, Tumor rejection antigen AB, tyrosinase, tyrosinase (oculocutaneous albinism IA)
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This monoclonal antibody shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this monoclonal antibody shows tyrosinase in melanotic as well as amelanotic variants. This monoclonal antibody is a useful marker for melanocytes and melanomas.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG2a κ
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Clone:
SPM360
Dilution:
Western Blot, Flow Cytometry, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Symbols:
TYR
Immunogen:
Recombinant tyrosinase protein (Uniprot: P14679 )
Quantity:
0.1 mL
Research Discipline:
Lipid and Metabolism
Gene ID (Entrez):
7299
Target Species:
Human
Form:
Purified

Img

Novus Biologicals

NBP234771B

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Antigen:
Biotin (Vitamin B7 or Vitamin H)
Classification:
Monoclonal
Conjugate:
Biotin
Gene Alias:
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG1 κ
Applications:
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Clone:
SPM375
Dilution:
Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Symbols:
__
Immunogen:
Biotinylated sheep immunoglobulin
Quantity:
0.1 mL
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
All species
Form:
Purified

Img

Novus Biologicals

NBP234771FR

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Antigen:
Biotin (Vitamin B7 or Vitamin H)
Classification:
Monoclonal
Conjugate:
DyLight 680
Gene Alias:
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG1 κ
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
SPM375
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Symbols:
__
Immunogen:
Biotinylated sheep immunoglobulin
Quantity:
0.1 mL
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
All species
Form:
Purified

Img

Novus Biologicals

NBP234771G

--

Antigen:
Biotin (Vitamin B7 or Vitamin H)
Classification:
Monoclonal
Conjugate:
DyLight 488
Gene Alias:
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG1 κ
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
SPM375
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Symbols:
__
Immunogen:
Biotinylated sheep immunoglobulin
Quantity:
0.1 mL
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
All species
Form:
Purified