NBP24461001

Nucleolin Antibody (NCL/902), Novus Biologicals™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP24461001-Each-of-1 In Stock ₹ 44,833.44

NBP24461001 - Each of 1

₹ 44,833.44

In Stock

Quantity

1

Base Price: ₹ 44,833.44

GST (18%): ₹ 8,070.019

Total Price: ₹ 52,903.459

Antigen

Nucleolin

Classification

Monoclonal

Concentration

0.2mg/mL

Dilution

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Gene Accession No.

P19338

Gene Symbols

NCL

Immunogen

Recombinant human NCL protein

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage

Store at 4C.

Isotype

IgG1 κ

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone

NCL/902

Conjugate

Unconjugated

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species

Mouse

Molecular Weight of Antigen

76 kDa

Quantity

0.1 mg

Research Discipline

Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez)

4691

Target Species

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form

Purified

Description

  • Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Img

Fischer Scientific

NBP24461001

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Gene Accession No.:
P19338

Gene Symbols:
NCL

Immunogen:
Recombinant human NCL protein

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
NCL/902

Conjugate:
Unconjugated

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
76 kDa

Quantity:
0.1 mg

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Img

Fischer Scientific

NBP24461002

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Gene Accession No.:
P19338

Gene Symbols:
NCL

Immunogen:
Recombinant human NCL protein

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
NCL/902

Conjugate:
Unconjugated

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
76 kDa

Quantity:
0.2 mg

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Img

Fischer Scientific

NBP24461200

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Gene Accession No.:
P19338

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
364-5

Conjugate:
Unconjugated

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
76 kDa

Quantity:
0.02 mg

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Img

Fischer Scientific

NBP24461201

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Gene Accession No.:
P19338

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
364-5

Conjugate:
Unconjugated

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
76 kDa

Quantity:
0.1 mg

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified