NBP24533300
TNF-alpha Antibody (J2D10), Novus Biologicals™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP24533300-Each-of-1 | In Stock | ₹ 22,930.08 |
NBP24533300 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 22,930.08
GST (18%): ₹ 4,127.414
Total Price: ₹ 27,057.494
Antigen
TNF-alpha
Classification
Monoclonal
Concentration
0.2 mg/mL
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.
P01375
Host Species
Mouse
Molecular Weight of Antigen
17 kDa
Quantity
0.02 mg
Research Discipline
Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez)
7124
Target Species
Human, Rat, Mouse (Negative)
Form
Purified
Applications
Flow Cytometry, SDS-Page, Immunofluorescence
Clone
J2D10
Conjugate
Unconjugated
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen
Recombinant human TNF-alpha
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage
Store at 4C.
Isotype
IgG1 κ
Related Products
Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence TNF-alpha Monoclonal specifically detects TNF-alpha in Human, Rat, Mouse (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence.
Compare Similar Items
Show Difference
Antigen: TNF-alpha
Classification: Monoclonal
Concentration: 0.2 mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.: P01375
Host Species: Mouse
Molecular Weight of Antigen: 17 kDa
Quantity: 0.02 mg
Research Discipline: Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez): 7124
Target Species: Human, Rat, Mouse (Negative)
Form: Purified
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Clone: J2D10
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen: Recombinant human TNF-alpha
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
TNF-alpha
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.:
P01375
Host Species:
Mouse
Molecular Weight of Antigen:
17 kDa
Quantity:
0.02 mg
Research Discipline:
Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez):
7124
Target Species:
Human, Rat, Mouse (Negative)
Form:
Purified
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Clone:
J2D10
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen:
Recombinant human TNF-alpha
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Antigen: TNF-alpha
Classification: Monoclonal
Concentration: 0.2 mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.: P01375
Host Species: Mouse
Molecular Weight of Antigen: 17 kDa
Quantity: 0.1 mg
Research Discipline: Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez): 7124
Target Species: Human, Rat, Mouse (Negative)
Form: Purified
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Clone: J2D10
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen: Recombinant human TNF-alpha
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
TNF-alpha
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.:
P01375
Host Species:
Mouse
Molecular Weight of Antigen:
17 kDa
Quantity:
0.1 mg
Research Discipline:
Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez):
7124
Target Species:
Human, Rat, Mouse (Negative)
Form:
Purified
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Clone:
J2D10
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen:
Recombinant human TNF-alpha
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Antigen: TNF-alpha
Classification: Monoclonal
Concentration: 0.2 mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.: P01375
Host Species: Mouse
Molecular Weight of Antigen: 17 kDa
Quantity: 0.2 mg
Research Discipline: Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez): 7124
Target Species: Human, Rat, Mouse (Negative)
Form: Purified
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Clone: J2D10
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen: Recombinant human TNF-alpha
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
TNF-alpha
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.:
P01375
Host Species:
Mouse
Molecular Weight of Antigen:
17 kDa
Quantity:
0.2 mg
Research Discipline:
Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Gene ID (Entrez):
7124
Target Species:
Human, Rat, Mouse (Negative)
Form:
Purified
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Clone:
J2D10
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Immunogen:
Recombinant human TNF-alpha
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Antigen: HNF-3 alpha/FoxA1
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot 2-4 ug/ml, Flow Cytometry 25 ug/ml, Immunohistochemistry 5 ug/ml, Immunocytochemistry/Immunofluorescence 1:25 - 1:50, Immunohistochemistry-Paraffin 5 ug/ml
Gene Accession No.: P55317
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 0.1 mg
Research Discipline: Breast Cancer, Cancer, Stem Cell Markers
Gene ID (Entrez): 3169
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone: 3B3NB
Conjugate: Unconjugated
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: forkhead box A1, Forkhead box protein A1, hepatocyte nuclear factor 3, alpha, hepatocyte nuclear factor 3-alpha, HNF-3A, HNF-3-alpha, HNF3ATCF-3A, MGC33105, TCF3A, Transcription factor 3A
Immunogen: Partial recombinant human FOXA1 protein (between amino acids 350-473) [UniProt# P55317].
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG2b κ
Antigen:
HNF-3 alpha/FoxA1
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot 2-4 ug/ml, Flow Cytometry 25 ug/ml, Immunohistochemistry 5 ug/ml, Immunocytochemistry/Immunofluorescence 1:25 - 1:50, Immunohistochemistry-Paraffin 5 ug/ml
Gene Accession No.:
P55317
Host Species:
Mouse
Molecular Weight of Antigen:
__
Quantity:
0.1 mg
Research Discipline:
Breast Cancer, Cancer, Stem Cell Markers
Gene ID (Entrez):
3169
Target Species:
Human
Form:
Purified
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
3B3NB
Conjugate:
Unconjugated
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
forkhead box A1, Forkhead box protein A1, hepatocyte nuclear factor 3, alpha, hepatocyte nuclear factor 3-alpha, HNF-3A, HNF-3-alpha, HNF3ATCF-3A, MGC33105, TCF3A, Transcription factor 3A
Immunogen:
Partial recombinant human FOXA1 protein (between amino acids 350-473) [UniProt# P55317].
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG2b κ