NBP247833B

NF-H Antibody (SPM203) - Azide and BSA Free, Novus Biologicals™

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

NF-H

Classification

Monoclonal

Concentration

1.0 mg/mL

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Gene Alias

200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Host Species

Mouse

Molecular Weight of Antigen

200 kDa

Quantity

0.2 mg

Research Discipline

Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Gene ID (Entrez)

4744

Target Species

Human, Mouse, Rat, Porcine, Chicken

Form

Purified

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), CyTOF

Clone

SPM203

Conjugate

Unconjugated

Formulation

PBS with No Preservative

Gene Symbols

NEFH

Immunogen

Triton-X 100 insoluble protein fraction of rat brain

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype

IgG1

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Description

  • NF-H Monoclonal specifically detects NF-H in Human, Mouse, Rat, Porcine, Chicken samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.

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Show Difference

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Fischer Scientific

NBP247833B

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Antigen:
NF-H

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Gene Alias:
200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Host Species:
Mouse

Molecular Weight of Antigen:
200 kDa

Quantity:
0.2 mg

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Gene ID (Entrez):
4744

Target Species:
Human, Mouse, Rat, Porcine, Chicken

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), CyTOF

Clone:
SPM203

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NEFH

Immunogen:
Triton-X 100 insoluble protein fraction of rat brain

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1

Img

Fischer Scientific

NBP247834A

--


Antigen:
NF-H

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Gene Alias:
200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Host Species:
Mouse

Molecular Weight of Antigen:
200 kDa

Quantity:
0.1 mg

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Gene ID (Entrez):
4744

Target Species:
Human, Mouse, Rat, Porcine, Chicken, Feline, Gerbil, Guinea Pig, Primate, Rabbit

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), CyTOF

Clone:
NE14

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NEFH

Immunogen:
Crude neurofilament preparation from porcine spinal cord

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1

Img

Fischer Scientific

NBP247834B

--


Antigen:
NF-H

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Gene Alias:
200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Host Species:
Mouse

Molecular Weight of Antigen:
200 kDa

Quantity:
0.2 mg

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Gene ID (Entrez):
4744

Target Species:
Human, Mouse, Rat, Porcine, Chicken, Feline, Gerbil, Guinea Pig, Primate, Rabbit

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), CyTOF

Clone:
NE14

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NEFH

Immunogen:
Crude neurofilament preparation from porcine spinal cord

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1

Img

Fischer Scientific

NBP247836A

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Antigen:
Ornithine Decarboxylase

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
ODCEC 4.1.1.17, ornithine decarboxylase, ornithine decarboxylase 1

Host Species:
Mouse

Molecular Weight of Antigen:
53 kDa

Quantity:
0.1 mg

Research Discipline:
Cell Cycle and Replication

Gene ID (Entrez):
4953

Target Species:
Human, Rat

Form:
Purified

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
ODC1/486

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
ODC1

Immunogen:
Recombinant human ODC-1 protein

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG2a κ