NBP247861A

Nucleolin Antibody (364-5) - Azide and BSA Free, Novus Biologicals™

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 NBP247861A-Each-of-1 In Stock ₹ 55,271.76

NBP247861A - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

Nucleolin

Classification

Monoclonal

Concentration

1.0 mg/mL

Dilution

Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species

Mouse

Molecular Weight of Antigen

76 kDa

Quantity

0.1 mg

Research Discipline

Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez)

4691

Target Species

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form

Purified

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone

364-5

Conjugate

Unconjugated

Formulation

PBS with No Preservative

Gene Symbols

NCL

Immunogen

Lysate of SU-DHL-1 Nuclei

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype

IgG1 κ

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Description

  • Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Compare Similar Items

Show Difference

Img

Fischer Scientific

NBP247861A

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
76 kDa

Quantity:
0.1 mg

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
364-5

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Img

Fischer Scientific

NBP247861B

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
76 kDa

Quantity:
0.2 mg

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
364-5

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Img

Novus Biologicals

NBP247862F

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
__

Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
364-5 + NCL/902

Conjugate:
FITC

Formulation:
PBS with 0.05% Sodium Azide

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Purification Method:
Protein A or G purified

Regulatory Status:
__

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Img

Novus Biologicals

NBP247862H

--


Antigen:
Nucleolin

Classification:
Monoclonal

Concentration:
__

Dilution:
Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)

Clone:
364-5 + NCL/902

Conjugate:
HRP

Formulation:
PBS with No Preservative

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Purification Method:
Protein A or G purified

Regulatory Status:
__

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ